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SEED-BORNE MYCOFLORA OF WHEAT, SORGHUM AND BARLEY

FAKHRUNNISA, M.H. HASHMI* AND A. GHAFFAR*

Abstract: Standard blotter and Deep Freezing methods were used to study the seed-borne mycoflora of 19 samples of wheat, 27 samples of sorghum and 14 samples of barley. A significant contamination with fungal genera was detected in analyzed samples. Fungi most frequently isolated and identified were Absidia sp., Alternaria alternata, Aspergillus sp., A. candidus, A. flavus, A. niger, A.sulphureus, Cephalosprium sp., Chaetomium globosum, Cladosporium herbarum, Curvularia lunata, Drechslera dematioidea, D. halodes, D. hawaiiensis, D. tetramera, Fusarium moniliforme, F. oxysporum, F. pallidoroseum, F. subglutinans, Nigrospora oryzae, Penicillium spp., Piptocephalis sp., Rhizoctonia solani, Rhizopus sp., Stemphylium sp., Syncephalastrum racemosum, Trichoderma hamatum, Trichothecium roseum and Ulocladium sp. This is the first report of Chaetomium globosum and D. hawaiiensis on wheat, A.sulphureus, Fusarium subglutinans, Nigrospora oryzae, Piptocephalis sp., Syncephalastrum racemosum and Trichoderma hamatum on sorghum and A. niger, Cephalosprium sp., Cladosporium herbarum, Drechslera dematioidea, D. tetramera, Trichothecium roseum, Stemphylium sp., and Ulocladium sp., are new records on barley. There does not appear to be any previous report of Absidia sp., Aspergillus sulphureus, Fusarium subglutinans and Rhizoctonia solani on wheat in Pakistan. Deep freezing method showed better results for isolation of Alternaria alternata, Cladosporium herbarum, Drechslera spp., and Fusarium spp.
 

Department of Botany, Khatoon-e-Pakistan Government Degree College for Women, Karachi, Pakistan.
Department of Botany, University of Karachi, Karachi, Pakistan.