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  Pak. J. Bot., 38(1): 85-88, 2006.

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  Updated: 09-07-09
   

UPTAKE OF NITRATE AND AMMONIUM ION BY CELL SUSPENSION CULTURES OF VIGNA RADIATA

SAADIA AFZAL, MUHAMMAD ZIA AND *M. FAYYAZ CHAUDHARY

Abstract: A study was conducted to examine the effect of pH on the uptake of NH4+ and NO3- by the cell suspension culture of Vigna radiata. Uptake of NH4+ was faster than NO3- under all conditions of the medium whereas uptake of NH4+ and NO3- both were affected by the pH of the medium. Maximum uptake of NH4+ was observed on pH 4.5. Uptake of NO3- was faster in the culture having pH 7.0. It is therefore concluded that pH of the medium influence the uptake of both NH4+ and NO3- in the cell suspension culture of Vigna radiata.
 


Department of Biological Sciences,Quaid-i-Azam University, Islamabad, Pakistan.
Corresponding Author: fayyazchaudhary@yahoo.com


   
         
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