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CALLUS INDUCTION AND REGENERATION IN SEED EXPLANTS OF RICE (ORYZA SATIVA CV. SWAT-II)

SHAZIA BANO, MUSARRAT JABEEN, FAZAL RAHIM AND IHSAN ILAHI

Abstract: Seeds of rice (Oryza sativa cv. Swat-II) were inoculated onto MS medium containing various concentrations of auxins and cytokinins. Excellent callus resulted on MS containing 2,4-D and Kn. However, this callus was non-embryogenic. Addition of tryptophan (TPN) @ 50 mg/l induced embryogenic callus. Increase in callus mass was obtained by a change in auxin and cytokinin level. Plantlet regeneration occurred on MS containing BAP at 0.5 mg/l in combination with IAA at a concentration of 0.2 mg/l. The plantlets were transferred to the field after hardening.

Department of Botany, University of Peshawar, Peshawar, Pakistan.