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TISSUE CULTURE STUDIES IN ORYZA SATIVA L. CVS. BASMATI 385 AND SUPER BASMATI

S. M. SAQLAN NAQVI, RAZIA SULTANA AND HAMID RASHEED*

Abstract: The main objective of the present study was to investigate and optimize callus induction frequency and regeneration in two rice cultivars viz., Basmati 385 and Super basmati and to monitor the effect of mannitol and sorbitol on regeneration. Callus induction was achieved by using different concentrations of 2, 4-D (1, 2, 3, 4 mgL^-1) on MS and N6 media with 2 mgL^-1producing the best results. Regeneration was studied only on MS medium containing BAP, NAA and 3% sucrose with or without either 3% mannitol or sorbitol. Super Basmati showed better results both for callusing and regeneration. Regeneration frequency decreased by the addition of either mannitol or sorbitol.
 

Department of Biochemistry, University of Arid Agriculture Rawalpindi, Pakistan.
National Agriculture Research Centre, Islamabad, Pakistan.