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INVERTASE PRODUCTION FROM A HYPERPRODUCING SACCHAROMYCES CEREVISIAE STRAIN ISOLATED FROM DATES

IKRAM-UL-HAQ AND SIKANDER ALI

Abstract: Invertase production by wild cultures of Saccharomyces cerevisiae isolated from dates available in local market is reported. Five hyperproducing yeast strains (>100 fold higher invertase activity) were kinetically analyzed for invertase production. Saccharomyces cerevisiae strain GCA-II was found to be a better invertase yielding strain among all other isolates. The values of Q_p and Y_p/s for GCA-II were highly significant as compared to other Saccharomyces cultures. The effect of sucrose concentration, rate of invertase synthesis, initial pH of fermentation medium and different organic nitrogen sources on the production of invertase under submerged culture conditions was investigated. Optimum concentrations of sucrose, urea and pH were 3 %, 0.2 % (w/v), and 6.0 respectively. The increase in the enzyme yield obtained after optimization of the cultural conditions was 47.7 %.
 

Department of Botany, Biotechnology Research Centre, Government College University, Lahore, Pakistan.