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GENETIC DIVERSITY AMONG RICE GENOTYPES OF PAKISTAN THROUGH RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) ANALYSIS

MUHAMMAD ARIF, SAMINA KOUSAR*, M. ASGHAR BAJWA*, ANJUMAN ARIF AND YUSUF ZAFAR

Abstract: Genetic diversity assessment among the 19 rice genotypes of Pakistan including 3 commercial Basmati cultivars viz., Basmati 370, Basmati 385 and Super Basmati was done using random amplified polymorphic DNA (RAPD) analysis. Out of 40 random decamer primers, 20 RAPD primers revealed polymorphism while 15 were monomorphic and no reaction was observed by using remaining 5 primers. OPI 7 generated maximum number of bands while OPZ 4 and OPZ 6 produced minimum fragments. The banding patterns obtained from the polymorphic primers were used to determine the genetic similarity coefficients which ranged from 0.42 to 0.85. Cluster analysis was performed using Unweighted Paired Group of Arithmetic Means (UPGMA) using the similarity coefficients. Cluster analysis resolved two major clusters among the 19 genotypes used. All the 3 commercial Basmati cultivars grouped together whereas the 15 rice genotypes clustered in another group. The remaining one genotype did not fall into any clusters and found to be distantly related to the rest of the genotypes. The results showed the potential of RAPD markers for genetic diversity assessment.
 

Plant Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad.
Department of Chemistry, University of Agriculture, Faisalabad.
Corresponding Author: marif@nibge.org