| Pak. J. Bot., 37(2): 399-406, 2005. | Back to Contents | ||||
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Updated: 09-07-09 | ||||
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RFLP-BASED RELATIONSHIP OF PAKISTANI ISOLATE OF BANANA BUNCHY TOP VIRUS WITH SOUTH PACIFIC VIRUS GROUP TAHIRA YASMIN, S.M. SAQLAIN NAQVI*, HUSSAIN SHAH AND SAIF KHALID Abstract: Restriction Fragment Length Polymorphism (RFLP) technique was employed
to investigate the relationship of Pakistani isolates of banana bunchy
top virus (BBTV) with Asia and South Pacific strains. The PCR amplified
product of BBTV DNA component I was digested with nine different restriction
endonucleases (Bam HI, EcoRI, EcoRV, HaeIII, HincII, HindIII, PstI,
RsaI and SmaI) and analyzed by agarose gel electrophoresis. HaeIII resulted
in three DNA fragments of 505, 232 and 188 base pairs while RsaI gave
four fragments of 310, 310, 250 and 200 base pairs. However, the DNA
remained undigested with rest of the endonucleases. On the basis of
electrophoretic patterns of restriction fragments, Pakistani BBTV isolate
is closely related to South Pacific virus group. Plant Virology Laboratory, Crop Diseases Research Program, National Agricultural Research Centre, Islamabad, Pakistan. Department of Biochemistry, University of Arid Agriculture, Rawalpindi, Pakistan. |
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