| Pak. J. Bot., 37(2): 389-398, 2005. | Back to Contents | ||||
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Updated: 09-07-09 | ||||
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COMPARISON OF PURIFICATION METHODS EMPLOYED FOR PAKISTANI ISOLATE OF BANANA BUNCHY TOP VIRUS TAHIRA YASMIN, HUSSAIN SHAH AND SAIF KHALID
Abstract:
Banana Bunchy Top Virus (BBTV) particles were purified employing various
purification methods. Substantial amount of virus particles were obtained
using tangential flow filters of millipore sizes of 10 and 0.16µm
and 100KDa/100mwco sequentially. The method was most successful and
gave high virus yield (75µg/ml) devoid of most host macromolecules.
Under electron microscopy (EM) isometric virus particles of 18-20 nm
in diameter were observed. The purified virus was used to raise polyclonal
antiserum against local isolate of BBTV giving an antibody titre of
1:6000 higher than other methods. Later Enzyme-linked immunosorbent
assay (ELISA) was also standardized for BBTV indexing. Plant Virology Lab., Crop Diseases Research Program, National Agriculture Research Centre, Park Road, Islamabad, Pakistan. |
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