Back to Contents

FREE FULL TEXT PDF

DNA FINGERPRINTING STUDIES OF SOME WHEAT (TRITICUM AESTIVUM L.) GENOTYPES USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) ANALYSIS

MUHAMMAD ASIF, MEHBOOB-UR-RAHMAN AND YUSUF ZAFAR*

Abstract: Random amplified polymorphic DNA (RAPD) analysis was employed to estimate genetic diversity/genetic similarity among nine wheat (Triticum aestivum L.) genotypes. Out of 25 random 10-mer primers surveyed, 18 primers detected polymorphism among all the wheat genotypes, while 7 primers produced monomorphic pattern. A total of 175 bands were amplified, 60.57% of which were polymorphic. Genetic similarity matrix was generated on the basis of Nei and Li's coefficients whose values ranged from 61.29% to 88.03%. The coefficients were used to make clusters using unweighted pair group method of arithmetic means (UPGMA). Wheat genotypes were grouped in two main clusters (A and B). However, CMH-771917/PKV1600 and VEE/Trap # 1 were the most distant genotypes, which were 61.29 to 76.53% and 66.01 to 78.81% genetically similar with the other genotypes, respectively. The information generated here along with the use of most advanced DNA fingerprinting techniques like AFLP, SCAR, SSR and ESTs would be helpful for future genome mapping programs as well as for the application of intellectual breeder rights in the country. The study will also work as indicator for wheat breeders to evolve varieties with diverse genetic background to achieve sustainability in wheat production in the country.
 

National Institute for Biotechnology and Genetic Engineering (NIBGE), P.O. Box 577, Jhang Road, Faisalabad, Pakistan

Corresponding Author: y_zafar@yahoo.com Fax: +92-41-651472