| Pak. J. Bot., 37(2): 249-262, 2005. | Back to Contents | ||||
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Updated: 09-07-09 | ||||
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RAPD CHARACTERIZATION OF SOMACLONAL VARIATION IN INDICA BASMATI RICE SULTANA RASHEED*, TAHIRA FATIMA, TAYYAB HUSNAIN, KHURRAM BASHIR AND SHIEKH RIAZUDDIN
Abstract:
Seeds of three cultivars of indica basmati rice viz., B-370, B-2000
and Super basmati were sterilized and incubated for 2, 4, 6 and 8 weeks
on callus induction medium. Fifteen interested regenerates were selected
on the basis of variation in important morphological characteristics.
DNAs from leaves of regenerated plants along with parental controls
were isolated and analyzed through PCR using 8 Random Amplified Polymorphic
DNA (RAPD) Primers. Four primers viz., S-13, S-19, R-17 and OPX-11 produced
detectable range of amplification products and 7 interesting clones
with different banding patterns were obtained. Index of genetic variation
was calculated and dendrograms were constructed using UPGMA method.
Genetic variation up to 45.2, 32.3, 32.4 & 35.3% was recorded after
8 weeks of incubation with primers S-13, S-19, R-17 and OPX-11 respectively.
Our study indicates that tissue culture generates a wide range of variation,
which is related with incubation time and is cultivar specific. Moreover,
RAPDs can successfully be used to explore such polymorphism within and
among different cultivars of basmati rice.
Department of Field Crops, National Center of Excellence in Molecular
Biology,
University of the Punjab, Lahore, Pakistan, 53700 Phone:
92-42-5423942 Fax: 92-42-516455 |
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