Back to Contents

FREE FULL TEXT PDF

SOURCES OF GENETIC RESISTANCE IN MUNGBEAN AND BLACKGRAM AGAINST URDBEAN LEAF CRINKLE VIRUS (ULCV)

MUHAMMAD BASHIR, ZAHOOR AHMAD AND ABDUL GHAFOOR

Abstract: In order to identify sources of genetic resistance in mungbean and blackgram (mash), 32 accessions (16 each of mungbean and blackgram) were evaluated under greenhouse conditions by sap inoculation method. The inoculated plants of each accession were also tested by enzyme-linked immunosorbent assay (ELISA) using polyclonal antiserum to ULCV to separate the resistant plants from susceptible ones. From mungbean, only five genotypes viz., VC-3960 (A-88), VC-3960 (A-89), 98-CMH-016, NM-2 and BRM-195 were found highly resistant to ULCV. These genotypes neither expressed disease symptoms nor virus was detected by ELISA. However, in case of 98MG-003 genotype, 10% plants were observed with mild symptoms of the leaf crinkle disease, and virus was also detected by ELISA at low titer. In case of blackgram (mash) only one genotype (VH-9440039-3) was found highly resistant and one ES-1 resistant to ULCV. The others were moderately susceptible to highly susceptible. In this study we report new genetic sources of resistance in mungbean and blackgram to ULCV which are available for breeding program to develop virus-resistant cultivars for commercial cultivation.
 

National Agricultural Research Centre, Park Road, Islamabad, Pakistan.