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IN VITRO PRODUCTION OF CORDYLINE TERMINALIS FOR COMMERCIALIZATION

SAIFULLAH KHAN* SHEEBA NAZ AND BUSHRA SAEED

Abstract: The current study envisages producing Cordyline plants on a large scale employing cost-effective In vitro protocols. The effect of various concentrations and types of plant growth regulators were observed to optimize the efficient media for propagation. Axillary buds excised from the greenhouse grown mother plants of Cordyline terminalis were cultured on MS medium supplemented with BAP (1.0-2.0 mg/L), Kinetin (2.0-4.0 mg/L) and NAA (0.1-0.5 mg/L). A rich solid mass of white-green callus was produced. Greatest number and length of shoots were produced in a medium containing kinetin (4.0 mg/L) and NAA (0.5mg/L). Rooting started on the same media when the plants had attained a height of 2.5 cm. Four weeks after a rich mass of roots were obtained the plants were transplanted individually in small clay pots and kept in close tunnels where the relative humidity was approximately 80%. Four weeks after acclimatization, the explants were shifted into green houses.
 

Plant Tissue Culture and Biotechnology Division, International Centre for Chemical Sciences,
H.E.J. Research Institute, University of Karachi 75270, Pakistan.