| Pak. J. Bot., 36(4): 751-756, 2004. | Back to Contents | ||||
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Updated: 09-07-09 | ||||
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AN ECONOMICAL AND EFFICIENT METHOD FOR MASS PROPAGATION OF IXORA COCCINEA SAIFULLAH KHAN, MARIAM IFTIKHAR AND BUSHRA SAEED
Abstract:
A simple and efficient micropropagation protocol was developed for Ixora
coccinea cv. Super King, a beautiful ornamental plant using shoot
tip and nodal segments as explants. The explants were cultured in Woody
Plant Medium (WPM) supplemented with 0.5 mg/L BAP incubated for 10 weeks
initially and subcultured onto fresh medium after every 4 weeks. The
proliferating clusters were cultured in WPM basal medium fortified with
Peptone (40 mg/l) and 3% sucrose for elongation of shoots, formation
of root initials and further proliferation of axillary shoots. Separation
and culturing of elongated shoots in fresh medium for 2 to 3 weeks yielded
7 to 8 cm long plantlets which were acclimatized in polytunnels. The
most suitable medium for rooting was half-WPM enriched with IBA at a
concentration of 0.05 mg/L. An increase in axillary shoot number of
up to 10 per shoot were observed. This exponential rise in plants was
very encouraging for the development of an efficient commercial micropropagation
system.
Plant Tissue Culture and Biotechnology Division, International Center
for Chemical Sciences, |
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