Pak. J. Bot., 36(4): 745-750, 2004. | Back to Contents | ||||
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Updated: 09-07-09 | ||||
RIGID BODY REFINEMENT OF WATER MEDIATED TRIPLE HELICAL STRUCTURE OF ß-D-1,3 XYLAN FROM PALMARIA PALMATA (L.) KUNTZE, RHODOPHYTA) NAHEED AKHTAR, SADAF NAEEM, WASEEM AHMED* AND M.A. HALEEM**
Abstract: The triple helical structure of ß-D-1,3 xylan from the cell wall
of Palmaria palmata (L.) Kuntze, (Rhodophyta) has been refined
using the rigid body refinement technique. Water molecule is also included
in the refinement. Using the least square rigid body refinement procedure
the structure has been refined to an R-value of 0.338. The agreement
between the calculated and observed structure factors is reasonably
good. The results indicate that the sugar rings have limited freedom
to rotate about 1, 3 glycosidic links and the molecule should be 9%
smaller in radius. The position of water molecule is also refined. The
loosely bound water follows the helical path. The present results support
the view that inter and intra chain hydrogen bonds are possible in the
water mediated structures. Biophysics Unit, Department of Biochemistry, University of Karachi, Karachi, Pakistan.
Federal Urdu University, Karachi. |
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