| Pak. J. Bot., 36(3): 629-634, 2004. | Back to Contents | ||||
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Updated: 09-07-09 | ||||
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PLANT REGENERATION BY SOMATIC EMBRYOGENESIS FROM CALLUS OF MATURE SEED EXPLANTS OF BREAD WHEAT (TRITICUM AESTIVUM L.) SAAD IMRAN MALIK1, HAMID RASHID2, TAYYABA YASMIN AND NASIR MAHMOOD MINHAS
Abstract:
The objective of
this study was to test genotypes and to evaluate media for effectiveness in promoting
whole plant regeneration by somatic embryogenesis in two wheat genotypes viz.,
Inqilab-91 and Pavon-76. Embryogenic callus was obtained by culturing seeds of
both varieties on gel-solidified Linsmaier and Skoog's (LS) medium with 3.5 mg/L
2,4-dichlorophenoxyacetic acid (2,4-D). The callus cultures were maintained by
subsequent subcultures onto fresh media at 4-5 weeks interval. The embryogenic
calli carefully cleared of any surrounding non-embryogenic callus were grown on
the gel-solidified basic Murashige and Skoog's (MS) medium with 3% (w/v) sucrose
after at least two subcultures. Seven different combinations of BAP (6-benzylaminopurine)
and IAA (Indole-3-acetic acid) i.e., 0 & 0.1, 0.5 & 0.1, 2.5 & 0.1,
5.0 & 0.1, 0.5 & 0, 2.5 & 0 and 5.0 & 0 mg/L, respectively, and
one control were assayed. Highest frequency of green spot formation and plant
regeneration (84% in Inqilab-91 and 52% in Pavon-76) was achieved at 0.5 &
0.1 mg/L of BAP and IAA, respectively. Higher concentrations of BAP in the media
proved to be toxic and fatal for callus in some cases. Significant difference
was observed among cultivars to callus in vitro and regenerate whole plants.
Department of Plant Breeding & Genetics,
University of Arid Agriculture, Rawalpindi, Pakistan. |
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