| Pak. J. Bot., 36(3): 549-556, 2004. | Back to Contents | ||||
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Updated: 09-07-09 | ||||
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IN VITRO CONSERVATION OF SUGARCANE (SACCHARUM OFFICINARUM L.) GERMPLASM MUHAMMAD SARWAR AND SADAR U. SIDDIQUI
Abstract: In order
to conserve the sugarcane germplasm in vitro, studies were undertaken at
the Plant Genetic Resources Institute, National Agricultural Research Centre,
Islamabad. The cultures were successfully established for var. Katha on MS medium
containing BA. Establishment of 8 other sugarcane varieties on the same medium
composition suggested its suitability for conservation of sugarcane germplasm.
In vitro conservation of var. Katha by osmotic stress showed that cultures
with 2% mannitol were healthy up to 165 days (showing 75% survival rate), while
the cultures with 1 and 3% mannitol showed 100% survival up to 105 days of storage.
The technique has great potential for the conservation of plant genetic resources
and more studies are required to further extend the subculture period by combining
the osmotic stress with incubation conditions management. National Agricultural Research Centre, Islamabad-45500, Pakistan. |
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