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KINETICS OF CITRATE OVER-PRODUCTION BY AN ASERGILLUS NIGER MUTANT RABt-10

RUBINA MAZHAR, ASAD-UR-REHMAN, *SIKANDER ALI AND IKRAM-UL-HAQ

Abstract: The present study deals with the kinetic investigation of the effect of initial sugar concentration and rate of citrate overproduction by a mutant strain of Aspergillus niger RABt-10 developed after UV treatment. The maximal production of citric acid (66.15±1.1 g/l) during the course of study was achieved using initial sugar concentration, 150 g/l while the time course was optimized at 168hrs after inoculation. All the kinetic parameters i.e., product and growth yield co-efficients (Yp/s, Yp/x and Yx/s in g/g), volumetric rates (Qp, Qs and Qx in g/g cells/h) and specific rate constants (qp, qs and qx in g/g/h) were highly significant.

Biotechnology Research Centre, Department of Botany, Government College University, Lahore, Pakistan.
Corresponding Author: alisbiotech@yahoo.com