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OPTIMIZATION OF CULTURAL CONDITIONS FOR THE PRODUCTION OF LIPASE BY SUBMERGED CULTURE OF RHIZOPUS OLIGOSPOROUS T^UV -31

TEHREEMA IFTIKHAR*, IKRAM-UL-HAQ AND MOHAMMAD MOHSIN JAVED

Abstract: The present study is concerned with the optimization of cultural conditions for the production of lipases by Rhizopus oligosporous T ^uv31. Studies were carried out in 250 ml Erlenmeyer flasks by submerged fermentation. A medium M 5 containing peptone (30 g/l), glucose (10 g/l), KH₂PO₄ (2 g/l), NaNO₃ (0.5 g/l), KCl (0.5 g/l), MgSO₄.7H₂O (0.5 g/l) was found suitable for optimum yield of lipase. The production of the enzymes reached maximum, 48 hours after inoculation at 30°C. The optimum inoculum size was 1.0 ml (4.63 X 10⁷ spores) of fermentation medium. Maximum extracellular and intracellular lipase activities were found to be 2.79 ± 0.15 U/ml and 1.66 ± 0.04 U/g.

Biotechnology Research Laboratories, Department of Botany, Government College University, Lahore, Pakistan.
Corresponding Author: pakaim2001@yahoo.com