| Pak. J. Bot., 35(4): 463-471, 2003. | Back to Contents | ||||
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Updated: 06-02-12 | ||||
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ABIOTIC FACTORS AFFECTING IN VITRO POLLEN GRAIN GERMINATION IN SAINFOIN (ONOBRYCHIS VICIIFOLIA SCOP.) CENGIZ SANCAK, SATI ÇÖÇÜ, KHALID MAHMOOD KHAWAR AND SEBAHATTIN ÖZCAN* Abstract: A reliable in vitro method for germination of sainfoin pollen grains has been developed. Highest pollen germination rate of 96% with mean pollen tube length of 223.5 µm was obtained on medium containing of 100 g/l sucrose, 200 mg/l KNO3, 150 mg/l H3BO3, 150 mg/l MgSO4, 150 mg/l Ca(NO3)2, 175 g/l PEG 4000 and stigma extract at pH 6.5. Department of Field Crops, Faculty of Agriculture, University of Ankara, 06110, Diskapi, Ankara, Turkey. |
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