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  Pak. J. Bot., 35(3): 409-414, 2003.

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  Updated: 06-02-12
   

SCREENING OF SUBSTRATES FOR MASS PRODUCTION OF BIOCONTROL AGENTS

SHAHNAZ DAWAR AND A. GHAFFAR

Abstract: Eleven different substrates viz., rice grain, sorghum grain, millet grain, cotton cake, mustard cake, wheat straw, rice straw, saw dust, sugarcane bagasse, sugarcane ash and wheat bran were used for the mass production of biocontrol agents viz., Paecilomyces lilacinus, Trichoderma harzianum, Gliocladium virens and Rhizobium meliloti. Rice grain, sorghum grain, millet grain and wheat bran were found suitable substrates for mass production of P. lilacinus and R. meliloti. Good growth of T. harzianum and G. virens was observed on sorghum grain followed by millet grain, rice grain, wheat bran, wheat straw, rice straw and sugar cane bagasse. Oil cakes, sugar cane ash and saw dust were found not suitable substrates for multiplication of biocontrol agents. The inoculum multiplied and stored in plastic bags remained viable for upto 360 days at 30°C.


Department of Botany, University of Karachi, Karachi-75270, Pakistan.


   
         
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