| Pak. J. Bot., 35(2): 209-217, 2003. | Back to Contents | ||||
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IN VITRO CALLUS INDUCTION, ITS PROLIFERATION AND REGENERATION IN SEED EXPLANTS OF WHEAT (TRITICUM AESTIVUM L.) VAR.LU-26S MOHAMMAD IHSAN SHAH, MUSSARAT JABEEN AND IHSAN ILAHI
Abstract: The present study was undertaken to evaluate the
most suitable concentration of growth regulators i.e., 2,4-D, IAA, BAP
and Kin for callus induction, its proliferation and regeneration in
seed explants of wheat (Triticum aestivum L) Lu-26S. Good callus
formation was obtained on MS medium containing 3.5-mg/l 2,4-D. A medium
containing 0.5 mg/l BAP in combination with 2.0 mg/l 2,4-D and 4.0mg/l
BAP alone also gave good response and a good callus proliferation was
obtained. Excellent regeneration was observed in a medium with 4.0 mg/l
BAP used alone and 2.0mg/l BAP in combination with 1.0 mg/l IAA. Department of Botany, University of Peshawar, Peshawar, Pakistan. |
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