| Pak. J. Bot., 33(3): 233-238, 2001. | Back to Contents | ||||
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PEG-MEDIATED SOMATIC HYBRIDIZATION STUDIESIN SUGARCANE (SACCHARUM SPP. HYBRID CVS. CoL-54 AND CP-43/33) FAHEEM AFTAB AND JAVED IQBAL
Abstract: Somatic hybridization studies were performed using leaf mesophyll
as well as homogeneous cell suspension-derived protoplasts. The optimum
results were achieved when leaf mesophyll protoplasts from cv. CP-43/33
and homogeneous cell suspension-derived protoplasts from cv. CoL-54
(at 2.0x10 5 ml-1 final density) were used. Polyethylene
glycol (PEG) at 35% level was found optimum for maximum protoplast agglutination
(32%). Maximum heterokaryon formation (17%) was obtained employing 50
mM l-1 CaCl2.2H20 at pH 10.5 as an
eluting solution. The hybridized protoplasts were cultured to monitor
the divisions and further growth. Department of Botany, University of the Punjab, Quaid-e-Azam Campus, Lahore 54590, Pakistan. |
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