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NUCELLAR REGENERATION AND POLYEMBRYONY OF CITRUS CULTIVARS

NAFEES ALTAF, E.K. MURWAT, I.A. BHATTI AND M. MOHSIN IQBAL

Abstract: Thirty four Citrus cultivars were studied for nucellar embryogenesis and for the extent of polyembryony per seed. Ovule weight had no correlation with the number of nucellar embryos. Also seed weight had no correlation with the number of embryos per seed. The maximum and minimum number of embryos per nucellus were in cvs. Kinnow (7.75) and Baramashi (2.23), respectively. Secondary somatic embryogenesis was also studied which was maximum in cv. Kinnow (8.40) and minimum in cv. Baramashi (2.90). Maximum and minimum polyembryony were in cultivars Minneola (7.50) and Orlando (2.75), respectively which indicated that nucellar embryogenesis was cultivar dependent. The soil survival after two months hardening process was maximum in cvs. Minneola (100 %), Shamber (100 %), Foster (100 %), Gada dehi (100 %) and minimum in cv. Baramashi (10.00%).
 

Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, Pakistan.