Pak. J. Bot., 33(2): 203-209, 2001. | Back to Contents | ||||
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Updated: 24-11-10 | ||||
IRREVERSIBLE INACTIVATION OF A TRANSGENE IN ARABIDOPSIS THALIANA BUSHRA MIRZA
Abstract: Plant transformation
is now a routine practice in many laboratories around the world. However it has
been observed that sometimes the introduced gene becomes inactive. This inactivation
could be reversible or irreversible. In this study the genetic behavior of an
inactive transgene was observed. For this purpose a wild type chlorophyll gene
CH-42 was introduced into a pale (ch-42) mutant Arabidopsis by
Agrobacterium mediated transformation. After transformation most of the plants
showed green wild type phenotype. However, some transgenic lines did not show
the phenotype of the CH-42 transgene and appeared pale in color. Four transgenic
lines showing inactive CH-42 transgene were selected to study the segregation
of the inactive transgene in the next generations. These transgenic lines with
inactive insert were crossed to the wildtype and the CH-42 mutant plants.
The F1 plants produced from these crosses were allowed to self fertilize and some
of the seeds of these F1 were germinated for analysis of the F2
generation. The results of the reciprocal crosses of all four inactive lines
were similar. The inactivation of transgene CH-42 was irreversible and
the cellular gene at the CH-42 locus did not have any effect on the expression
of the transgene. Department of Biological Sciences Quaid-i- Azam University, Islamabad, Pakistan. |
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