Pak. J. Bot., 33(2): 173-179, 2001. | Back to Contents | ||||
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Updated: 24-11-10 | ||||
CHARACTERIZATION OF MUSTARD [BRASSICA JUNCEA (L.) CZERN. & COSS.] GERMPLASM BY SDS-PAGE OF TOTAL SEED PROTEINS M. A. RABBANI, A.A. QURESHI, M. AFZAL, R. ANWAR AND S. KOMATSU*
Abstract: A comparative study of total seed storage
protein was carried out by sodium dodecyl sulfate polyacrylamide gel electrophoresis
(SDS-PAGE) to characterize oilseed mustard [Brassica juncea (L.) Czern.
& Coss.] germplasm from Pakistan. Oilseed collections from Pakistan as well
as oilseed cultivars from diverse origin were not differentiated from each other
nor were vegetable cultivars found to be distinct from one another on the basis
of their seed protein patterns. Eight types of protein were recognized based on
the banding patterns of 52 accessions. Relative inter-type relationships of protein
patterns were estimated using Jaccard's similarity index and a dendrogram showing
the hierarchical clustering was constructed by unweighted pair-group method with
arithmetic averages (UPGMA). The clustering of eight protein types generally agreed
with our previous classification and the limited data already available on inter-accession
relationships in oilseed mustard based on morphological traits and RAPD analysis.
The differences between the profiles of accessions 'PAK-85835', 'PAK-85839' and
'PAK-85910' supported the idea that they were separate species from B. juncea.
The results showed that the technique of SDS-PAGE applied to seed proteins was
not feasible to distinguish the closely related oilseed collections and cultivars
from each other as they were characterized by the same banding pattern and formed
a common gene-pool. However, seed proteins were useful to discriminate B. juncea
and B. campestris. It was also possible to distinguish the oilseed mustard
from the vegetable form. Future studies should involve a greater number of local
accessions of oilseed mustard from other parts of Pakistan, and those of vegetable
and condiment forms to further elucidate the situation.
Plant Genetic Resources Institute, National Agricultural Research Center,
Islamabad 45500, Pakistan. |
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