Back to Contents

FREE FULL TEXT PDF

PROTOPLAST ISOLATION IN SUGARCANE

IMTIAZ AHMED KHAN, ABDULLAH KHATRI , MUHAMMAD ASLAM JAVED, SHAMIM HUSSAIN SIDDIQUI, MAQBOOL AHMAD, NAZIR AHMED DAHAR, MUHAMMAD HUSSAIN KHANZADA AND RAZIULLAH KHAN

Abstract: A protocol was established for isolation of sugarcane protoplasts, cell wall regeneration and cell division. Protoplast were isolated from cell suspension initiated from fresh leaf calli and produced on agar medium by enzymatic treatment, 4% cellulase, 2% macerozyme, 1% pectinase and 0.7M mannitol at pH 5.4 under a defined conditions.
 

Nuclear Institute of Agriculture, Tandojam, Sindh, Pakistan.