Pak. J. Bot., 33(2): 117-124, 2001. | Back to Contents | ||||
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Updated: 24-11-10 | ||||
EFFECT OF SOME SALTS ON IN VITRO GROWTH OF FUSARIUM SOLANI HINA FIRDOUS AND SALEEM SHAHZAD
Abstract: Effect of different salts viz., sea salt, NaCl , CaCl2,
NaNO3, MgSO4, MgCl2 and KCl was studied
on in vitro growth of Fusarium solani by food poison method.
Growth of F. solani was enhanced as compared to control in potato
sucrose agar (PSA) medium containing sea salt @ 20,000 ppm but suppressed
where sea salt was used @ 60,000 ppm or more. No conidia were produced
in 100,000 ppm of sea salt. Similarly, F. solani showed enhanced
growth in NaCl at 10,000 and 20,000 ppm and declined with increasing
conc. from 30,000 to 100,000 ppm. Growth of F. solani also increased
significantly with increasing conc. of MgSO4 up to 40,000
ppm but declined thereafter. Use of NaNO3 @ 10,000 to 70,000
ppm showed significant increase in growth with massive cottony white
mycelium as compared to control. Growth of F. solani declined
gradually compared to control with increasing conc. of MgCl2
from 20,000 ppm to 100,000 ppm. Similarly, use of KCl @ 20,000 to 40,000
ppm showed gradual decline in growth of F. solani whereas @ 50,000 ppm
or more, growth started after 7-days of inoculation. Amendment of PSA
with CaCl2 showed gradual decline in growth of F. solani
with increasing conc. of CaCl2. Chlamydospores were produced
only where NaNO3 was used @ 10,000 to 40,000 ppm. Plant Disease Research Lab., Department of Botany, University of Karachi, Karachi-75270, Pakistan. |
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