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STUDIES ON AQUEOUS EXTRACTS OF THREE GREEN ALGAE AS AN ELICITOR OF PLANT DEFENCE MECHANISM

FATIMA BI AND SEEMA IQBAL

Abstract: Codium eiongatum, Cauierpa texijlora and Viva lactuius (green algae) were collected from Karachi coast. High Molecular Weight Crude Elicitor Preparation (HMWCEP) of these plants were obtained by sequential extraction of H₂O, dilute NaOH and HCl, ethanol precipitation and freeze drying. Results showed hat yields were high in NaOH extracts ranging from 4.3 - 69.6%. These HMWCEP were chemically anaysed for total sugar, protein, SO₄ group and uronic acid content. Elicitor activity was determined in terms of induced browning in Cicer arietinum (chickpea) tissues. Amongst the three algae HMWCEP of Codium elongatum was found the most active elicitor and produced maximum browning after 24 hour incubation at 25^°C.

Pakistan Council of Scientific and Industrial Research Laboratories, Karachi, Pakistan.