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MANIPULATION OF INTERNODAL SEGMENTS OF F₁ TOMATO HYBRIDS RAISED IN VITRO UNDER DIFFERENT REGIMES OF PLANT GROWTH REGULATORS

SHAKEEL AHMAD JATOI, MUSHTAQ AHMAD* AND HIDAYAT ULLAH SUPPAL*

Abstract: The relative morphogenetic response of internodal segments of tomato hybrids viz., Bornia and Royesta was studied at varying concentration of BAP, Kn, NAA and IAA. NAA @ 3 uM/I proved to be better than IAA @ 3 uMI in combination with different PGR regimes of both BAP (0, 3, 6, 9 uM/I) as well as Kn (0, 6, 12, 18 uM/I) exhibiting maximum callus formation in both the hybrids whereas, its response for root regeneration was not satisfactory. The influence of IAA in combination with both cytokinins was comparatively less callogenetic but for root regeneration its response was better than NAA. A little shoot regeneration in both hybrids was observed when callus was subjected to regeneration medium containing 2 mg/l and 0.1 mg/l of IBA.

Department of Horticulture, Faculty of Agriculture, Gomal University, D.I. Khan, Pakistan.
Tissue Culture Lab, NARC, Islamabad, Pakistan.