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  Pak. J. Bot., 30(2): 253-261, 1998.

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  Updated: 08-04-11
   

IN VITRO MUTAGENESIS IN SUGARCANE

IMTIAZ AHMED KHAN, ABDULLAH KHATRI, MAQBOOL AHMAD, SHAMIM HUSSAIN SIDDIQUI, GHULAM SHAH NIZAMANI, MOHAMMAD HUSSAIN KHANZADA, NAZIR AHMED DAHAR AND RAZIULLAH KHAN.

Abstract: Two sugarcane clones viz., AEC81-8415 and BL4 were tested for in vitro mutagenesis using irradiation doses of 0, 10, 20, 30, 40 and 50Gy. Maximum callus proliferation and shoot regeneration was observed in 10Gy in both clones with linear decrease in callusing and regeneration potential when the irradiation dose was increased upto 50Gy. Better response in root development was observed in MS medium containing 6% sucrose and 1mg/1lBA. Data on quantitative and qualitative traits were also recorded.


Atomic Energy Agricultural Research Centre, Tando jam, Sindh, Pakistan.


   
         
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