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ISOZYME POLYMORPHISM IN ASCOCHYTA RABIEI ISOLATES FROM PAKISTAN

S. HUSSAIN* AND W. BARZ

Abstract: Fifteen isolates of Ascochyta rabiei causing blight of chickpea (Cicer arietinum) were collected from Pakistan and analyzed for esterase, acid phosphatase, alcohol dehydrogenase, lactate dehydrogenase, malate dehydrogenase, glutamate dehydrogenase and fumarase. Only two enzymes viz., esterase and acid phosphatase produced qualitative as well as quantitative differences among the isolates of A. rabiei. Six electrophoretic phenotypes were developed in case of acid phosphatise, whereas isoelectric focusing of the protein extracts revealed simpler banding patterns for the esterase activity on gel. No correlation between isozyme patterns and the pathogenic groups were established on the basis of aggressiveness.

Institute fir Biochemei und Biotecnology der Pflanzen, University Munster, Munster-48143, Germany.

Corresponding Author: Pluses Programme, National Agriculture Research Centre, Islamabad-45500, Pakistan.