Pak. J. Bot., 28(2): 203-206, 1996. | Back to Contents | ||||
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Updated: 23-12-10 | ||||
HIGH FREQUENCY ADVENTITIOUS PLANT REGENERATION FROM RADICLE EXPLANTS OF AEGLE MARMELOS CORR. R. ISLAM, A. AHAD, M.H. RAHMAN, M. HOSSAIN AND O.I. JOARDER Abstract: A procedure was developed which allows high frequency adventitious shoot regeneration from radicle tissues of Aegle marmelos. Adventitious buds were initiated on Murashige & Skoog's (MS) medium containing various combinations of benzyl adenine (BA) and naphthaleneacetic acid (NAA). The medium containing 1.0 mg/1 BA and 0.2 mg/1 NAA produced highest number of shoots per explant with maximum frequency of regeneration. Shoots were elongated by transferring explants with shoot buds to a medium with a low concentration (0.1 mg/1) of BA. The shoots grown in medium containing 25 mg/l indolebutyric acid (IBA) for one week, when transferred to basal medium produced adventitious roots. Maximum rooting (80%) with 3-6 roots per shoot was achieved. Department of Botany, University of Rajshahi, Rajshahi 6205, Bangladesh. |
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