| Pak. J. Bot., 28(2): 167.172, 1996. | Back to Contents | ||||
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TRANSFORMATION OF ALFALFA (MEDlCAGO SATIVA L.) PLANTS WITH GUS MARKER CONTAINING INTRON SAIRA SHABNAM, YUSUF ZAFAR AND KAUSER A. MALIK Abstract: Alfalfa (Medicago sativa L. cv Regen SY) plants were regenerated from roots induced by inoculation of leaf discs with Agrobacterium rhizogenes containing the vector p35S GUS INT. The DNA transformed to the plant genome with T-DNA based vector contained genes which encoded neomycin phosphotransferase (NPT II), that confers resistance to kanamycin and intron containing ß-D glucuronidase (GUS) gene. Histochemical staining for all the tested kanamycin resistant plants exhibited GUS activity and some differences in the phenotypic characters were also noted in the transformed and untransformed plants. National Institute for Biotechnology & Genetic Engineering (NIBGE): P. O. Box 577, Faisalabad, Pakistan. |
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