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COMPARISON OF SALT STRESS RESPONSIVE PROTEINS IN ATRIPLEX AMNICOLA ANTIBODIES AND TWO ECOTYPES OF LEPTOCHLOA FUSCA (L.) KUNTH.

A. NASEER AZIZ, SYED H. ALl, NASIR AHMAD, JAVED A. QURESHI AND KAUSER A. MALIK

Abstract: Three salt tolerant plant types viz., Atriplex amllicola, Leptochloa fusca (L) Kunth. (Pakistani origin) and Leptochloa fusca (L) Kunth (Australian origin) were used for a comparative study of altered gene expression under salt stress. The plants were aseptically grown in hydroponic medium and exposed to increasing levels of salt starting from 50mM NaCI to 500mM NaCI. Under these stress treatments there was variable expression of a number of proteins as revealed by SDS-PAGE. Specific activities of plant GDH, GOGATand GSin response to salt stress were also determined. Klebsiella sp., NIAB-l, adiazotroph isolated from roots of Leptochloa fusca (L.) Kunth was also exposed to 50mM NaCI to 1000mM NaCI treatments, to examine salt responsive protein profiles. Blots of protein profiles from the three plant types were probed with antibodies raised against p-20 of Atriplex amnicola and p-26 of Klebsiella sp., NIAB-1, to reveal common epitopes among the salt responsive proteins.

Basic Biology Division, National Institute for Biotechnology and Genetic Engineering, P.G.Box 577, Faisalabad, Pakistan.