Pak. J. Bot., 25(1): 21-28, 1993.

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			Updated: 27-12-10
	*EFFECT OF AQUEOUS EXTRACTS AND LEACHATES OF ANTHERS OF CERATONIA SILIQUA* L., ON THE INHIBITION OF LETTUCE SEED GERMINATION M. ISHAQ KHAN AND BUSHREEN JAHAN Abstract:** Aqueous extracts and leachates of young, mature, and senesced anthers of Ceratonia siliqua were tested for their inhibition of lettuce seed gemlination. Only senesced anthers was found to contain substantial amounts of germination inhibitors. Isolation of the inhibitors from the anthers revealed the inhibitors to be soluble in water, methanol, ethanol, ethyl acetate and was acidic in nature. Kinetin and GA₃ were found to counteract the inhibition of the aqueous extract and leachates of the senesced anthers. Little,loss in the inhibitory activity of the aqueous extract occured even after subjecting the anthers to the highest soil temperature of 80°C for 60 minutes, which indicated that the inhibitors were not proteinaceous. Department of Botany, University of Karachi, Karachi 75270, Pakistan

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