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POLLEN EMBRYOGENESIS IN THE CULTURED ANTHERS OF NICOTIANA-TABACUM CV
VIRGINICA OBTAINED FROM INVIVO AND INVITRO PRODUCED FLOWER BUDS
KHATOON K
Abstract: The performance of anthers from in vitro produced flower buds and those
derived from glasshouse-grown plants was compared. In liquid medium
anthers of all types exhibited some degree of browning. In vivo produced
anthers were moderately prone to browning irrespective of the presence
or absence of hormones in the culture medium. Browning in anthers
derived from in vitro produced flower buds was far more in hormone-free
media. The anther response based on the number of anthers incubated was
much higher in hormone free media than in the media containing hormones.
In vitro produced anthers exhibited a strong dependence of anther
response to the stage of pollen development at excision. The
productivity of the in vitro produced anthers was greater in the hormone
supplemented media whereas the in vivo produced anthers did so in the
basal medium.
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